Overview

  • Product nameAnti-SMCR8 antibody
    See all SMCR8 primary antibodies
  • Description
    Rabbit polyclonal to SMCR8
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to internal sequence amino acids 279-396 of Human SMCR8.

  • Positive control
    • Human rectum tissue; RT 4, U 251 MG, and Human Tonsil lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

Anti-SMCR8 antibody images

  • ab126821 at 1/150 dilution staining SMCR8 in Paraffin-embedded Human Rectum tissue by Immunohistochemistry.
  • All lanes : Anti-SMCR8 antibody (ab121682) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique
  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nuclei but not nucleoli using ab121682.
    Recommended treatment:
    Fixation/Permeabilization: PFA/Triton X-100

References for Anti-SMCR8 antibody (ab121682)

This product has been referenced in:
  • Yang M  et al. A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy. Sci Adv 2:e1601167 (2016). Mouse . Read more (PubMed: 27617292) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"