Overview

  • Product nameAnti-Smek1 antibody
    See all Smek1 primary antibodies
  • Description
    Rabbit polyclonal to Smek1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 770-820 of Human Smek1 (NP_115949.3)

  • Positive control
    • Human Breast Carcinoma, Human Colon Carcinoma, Human Lung Adenocarcinoma, Human Non-Small Cell Lung Cancer, Human Placenta, Human Prostate Carcinoma, Human Stomach Adenocarcinoma, Human Testicular Seminoma, Mouse Renal Cell Carcinoma, Mouse Teratoma

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with Tris-EDTA pH9.0 buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionRegulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
    • Sequence similaritiesBelongs to the SMEK family.
      Contains 1 WH1 domain.
    • Cellular localizationCytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. In interphase localized in the cytoplasm and in the nucleus (with higher levels). During metaphase located in pericentriolar regions.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLFL1 antibody
      • KIAA2010 antibody
      • MEK1 SUPPRESSOR 1 antibody
      • MSTP033 antibody
      • P4R3A_HUMAN antibody
      • PP4R3 ALPHA antibody
      • PP4R3A antibody
      • PPP4R3A antibody
      • PROTEIN PHOSPHATASE 4, REGULATORY SUBUNIT 3, ALPHA antibody
      • Serine/threonine-protein phosphatase 4 regulatory subunit 3A antibody
      • SMEK homolog 1 antibody
      • SMEK homolog 1, suppressor of mek1 (Dictyostelium) antibody
      • smek1 antibody
      • SMK1 antibody
      see all

    Anti-Smek1 antibody images

    • ab91618, at 1/250 dilution, staining Smek1 in formalin-fixed paraffin-embedded Human stomach carcinoma tissue by Immunohistochemistry using DAB staining.
    • ab91618, at 1/250 dilution, staining Smek1 in formalin-fixed paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry using DAB staining.
    • ab91618, at 1/250 dilution, staining Smek1 in formalin-fixed paraffin-embedded Mouse teratoma tissue by Immunohistochemistry using DAB staining.
    • ab91618, at 1/250 dilution, staining Smek1 in formalin-fixed paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry using Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.

    References for Anti-Smek1 antibody (ab91618)

    ab91618 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab91618.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"