Mouse, Human Predicted to work with:
Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within C terminal amino acids 770-820 of Human Smek1 (NP_115949.3)
Human Breast Carcinoma, Human Colon Carcinoma, Human Lung Adenocarcinoma, Human Non-Small Cell Lung Cancer, Human Placenta, Human Prostate Carcinoma, Human Stomach Adenocarcinoma, Human Testicular Seminoma, Mouse Renal Cell Carcinoma, Mouse Teratoma
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with Tris-EDTA pH9.0 buffer will enhance staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRegulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
Sequence similaritiesBelongs to the SMEK family. Contains 1 WH1 domain.
Cellular localizationCytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. In interphase localized in the cytoplasm and in the nucleus (with higher levels). During metaphase located in pericentriolar regions.
ab91618, at 1/250 dilution, staining Smek1 in formalin-fixed paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry using Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 secondary antibody at a dilution of 1/100.
References for Anti-Smek1 antibody (ab91618)
has not yet been referenced specifically in any publications.
Publishing research using ab91618? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"