Overview

  • Product nameAnti-Smg1 antibody
    See all Smg1 primary antibodies
  • Description
    Rabbit polyclonal to Smg1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 3600 - C-terminus of Human Smg1.

    (Peptide available as ab31816.)

  • Positive control
    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab30916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: Use at 1:200 (See Abreview Feb 20 2007).
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 340 kDa (predicted molecular weight: 340 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
    • Tissue specificityWidely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 1 HEAT repeat.
      Contains 1 PI3K/PI4K domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localizationNucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 61E3.4 antibody
      • ATX antibody
      • hSMG-1 antibody
      • hSMG1 antibody
      • KIAA0421 antibody
      • Lambda interacting protein antibody
      • Lambda-interacting protein antibody
      • Lambda/iota protein kinase C interacting protein antibody
      • Lambda/iota protein kinase C-interacting protein antibody
      • LIP antibody
      • Phosphatidylinositol 3 kinase related kinase antibody
      • Phosphatidylinositol 3 kinase related protein kinase antibody
      • PI 3 kinase related kinase SMG 1 antibody
      • Serine/threonine protein kinase SMG1 antibody
      • Serine/threonine-protein kinase SMG1 antibody
      • SMG-1 antibody
      • smg1 antibody
      • SMG1_HUMAN antibody
      see all

    Anti-Smg1 antibody images

    • All lanes : Anti-Smg1 antibody (ab30916) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
      Lane 2 : Jurkat whole cell lysate (ab7899) at 10 µg
      Lane 3 : A431 whole cell lysate (ab7909) at 20 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size : 340 kDa
      Observed band size : 340 kDa
    • ab30916 (1/200) staining Smg1 by ICC/IF in assynchronous HeLa Cells (green). Cells were countersatined with DAPI (red) in order to show the nucleus. Secondary antibody: Goat anti-Rabbit conjugated to Cy3®. Please refer to abreview for further details.

      See Abreview

    • ICC/IF image of ab30916 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30916, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml

    References for Anti-Smg1 antibody (ab30916)

    This product has been referenced in:

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 20 µg
    Specification HeLa
    Gel Running Conditions Reduced Denaturing (4-12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 12 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa and 293T cells)
    Loading amount 10 µg
    Specification HeLa and 293T cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    Submitted May 02 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Feb 20 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"