• Product nameAnti-Smg1 antibody
    See all Smg1 primary antibodies
  • Description
    Rabbit polyclonal to Smg1
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1625-1675 of Human Smg1

  • Positive control
    • Nuclear extract from HeLa cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 100mM Sodium chloride, 10mM PBS, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab70439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 410 kDa.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionSerine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
    • Tissue specificityWidely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 1 HEAT repeat.
      Contains 1 PI3K/PI4K domain.
    • Post-translational
    • Cellular localizationNucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 61E3.4 antibody
      • ATX antibody
      • hSMG-1 antibody
      • hSMG1 antibody
      • KIAA0421 antibody
      • Lambda interacting protein antibody
      • Lambda-interacting protein antibody
      • Lambda/iota protein kinase C interacting protein antibody
      • Lambda/iota protein kinase C-interacting protein antibody
      • LIP antibody
      • Phosphatidylinositol 3 kinase related kinase antibody
      • Phosphatidylinositol 3 kinase related protein kinase antibody
      • PI 3 kinase related kinase SMG 1 antibody
      • Serine/threonine protein kinase SMG1 antibody
      • Serine/threonine-protein kinase SMG1 antibody
      • SMG-1 antibody
      • smg1 antibody
      • SMG1_HUMAN antibody
      see all

    Anti-Smg1 antibody images

    • Lanes 1 - 2 : Anti-Smg1 antibody (ab70439) at 1 µg/ml
      Lanes 3 - 4 : Anti-Smg1 antibody (ab70439) at 0.4 µg/ml
      Lanes 5 - 6 : Anti-Smg1 antibody (ab70439) at 0.1 µg/ml

      Lane 1 : Nuclear extract from HeLa cells. at 5 µg
      Lane 2 : Nuclear extract from HeLa cells. at 15 µg
      Lane 3 : Nuclear extract from HeLa cells. at 15 µg
      Lane 4 : Nuclear extract from HeLa cells. at 50 µg
      Lane 5 : Nuclear extract from HeLa cells. at 15 µg
      Lane 6 : Nuclear extract from HeLa cells. at 50 µg

      developed using the ECL technique

      Predicted band size : 410 kDa
      Observed band size : >177 kDa (why is the actual band size different from the predicted?)

    References for Anti-Smg1 antibody (ab70439)

    ab70439 has not yet been referenced specifically in any publications.

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