Overview

  • Product nameAnti-Smg1 antibody
    See all Smg1 primary antibodies
  • Description
    Rabbit polyclonal to Smg1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    Synthetic peptide corresponding to a region between residues 2300 and 2350 of Human Smg1 (Q96QV0)

  • Positive control
    • Human breast adenocarcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified using an epitope specific to Smg1 immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionSerine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
    • Tissue specificityWidely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
    • Sequence similaritiesBelongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 1 HEAT repeat.
      Contains 1 PI3K/PI4K domain.
    • Post-translational
      modifications
      Autophosphorylated.
    • Cellular localizationNucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 61E3.4 antibody
      • ATX antibody
      • hSMG-1 antibody
      • hSMG1 antibody
      • KIAA0421 antibody
      • Lambda interacting protein antibody
      • Lambda-interacting protein antibody
      • Lambda/iota protein kinase C interacting protein antibody
      • Lambda/iota protein kinase C-interacting protein antibody
      • LIP antibody
      • Phosphatidylinositol 3 kinase related kinase antibody
      • Phosphatidylinositol 3 kinase related protein kinase antibody
      • PI 3 kinase related kinase SMG 1 antibody
      • Serine/threonine protein kinase SMG1 antibody
      • Serine/threonine-protein kinase SMG1 antibody
      • SMG-1 antibody
      • smg1 antibody
      • SMG1_HUMAN antibody
      see all

    Anti-Smg1 antibody images

    • ab82548, at a 1:250 dilution, staining human Smg1 in breast adenocarcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    References for Anti-Smg1 antibody (ab82548)

    ab82548 has not yet been referenced specifically in any publications.

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    Thank you for your call today. As we discussed, please forward any protocol information that you can provide and I will be happy to refund this purchase for you. In particular, we would like to know: 1) How was the tissue fixed? ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"