Overview

  • Product nameAnti-SMG7 antibody
    See all SMG7 primary antibodies
  • Description
    Rabbit polyclonal to SMG7
  • SpecificityThis antibody detects endogenous levels of total SMG7 protein.
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) from an internal region of SMG7.

  • Positive control
    • WB: Extracts from HeLa cells, Jurkat cells, 293 cells and HUVEC cells. ICC/IF: HepG2 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • FunctionPlays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.
  • Sequence similaritiesContains 2 TPR repeats.
  • Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic, and nuclear. Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • breast cancer-associated antigen SGA-56M antibody
    • C1orf16 antibody
    • EST1 like protein C antibody
    • EST1 telomerase component homolog C antibody
    • EST1-like protein C antibody
    • EST1C antibody
    • ever shorter telomeres 1C antibody
    • FLJ23717 antibody
    • hSMG-7 antibody
    • nonsense mediated mRNA decay factor (C. elegans) antibody
    • Protein SMG7 antibody
    • SGA56M antibody
    • SMG 7 antibody
    • SMG-7 homolog antibody
    • Smg7 antibody
    • SMG7_HUMAN antibody
    see all

Anti-SMG7 antibody images

  • All lanes : Anti-SMG7 antibody (ab64847) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells at 5 µg
    Lane 2 : Extracts of Jurkat cells at 5 µg
    Lane 3 : Extracts of 293 cells at 5 µg
    Lane 4 : Extracts of HUVEC cells at 5 µg
    Lane 5 : Extracts of HUVEC cells plus blocking peptide at 5 µg


    Predicted band size : 126 kDa
    Observed band size : 126 kDa
  • Immunofluorescence analysis of HepG2 cells, using ab64847 (1/500).

    The left panel is in the absence of blocking peptide, whereas the right panel is in the presence of blocking peptide.

References for Anti-SMG7 antibody (ab64847)

ab64847 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. These antibodies have both been tested in a straight ELISA, not in a sandwich ELISA and do not have matched pairs. The detection limit of ab77962 is 1/2,000, for ab64857 it is 1/20,000. Please let me know if you have any fu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"