Overview

  • Product name
  • Description
    Rabbit polyclonal to SMG7
  • Specificity
    This antibody detects endogenous levels of total SMG7 protein.
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) from an internal region of SMG7.

  • Positive control
    • WB: Extracts from HeLa cells, Jurkat cells, 293 cells and HUVEC cells. ICC/IF: HepG2 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • Function
    Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.
  • Sequence similarities
    Contains 2 TPR repeats.
  • Cellular localization
    Cytoplasm. Nucleus. Predominantly cytoplasmic, and nuclear. Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • breast cancer-associated antigen SGA-56M antibody
    • C1orf16 antibody
    • EST1 like protein C antibody
    • EST1 telomerase component homolog C antibody
    • EST1-like protein C antibody
    • EST1C antibody
    • ever shorter telomeres 1C antibody
    • FLJ23717 antibody
    • hSMG-7 antibody
    • nonsense mediated mRNA decay factor (C. elegans) antibody
    • Protein SMG7 antibody
    • SGA56M antibody
    • SMG 7 antibody
    • SMG-7 homolog antibody
    • Smg7 antibody
    • SMG7_HUMAN antibody
    see all

Images

  • All lanes : Anti-SMG7 antibody (ab64847) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells at 5 µg
    Lane 2 : Extracts of Jurkat cells at 5 µg
    Lane 3 : Extracts of 293 cells at 5 µg
    Lane 4 : Extracts of HUVEC cells at 5 µg
    Lane 5 : Extracts of HUVEC cells plus blocking peptide at 5 µg


    Predicted band size : 126 kDa
    Observed band size : 126 kDa
  • Immunofluorescence analysis of HepG2 cells, using ab64847 (1/500).

    The left panel is in the absence of blocking peptide, whereas the right panel is in the presence of blocking peptide.

References

ab64847 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us. These antibodies have both been tested in a straight ELISA, not in a sandwich ELISA and do not have matched pairs. The detection limit of ab77962 is 1/2,000, for ab64857 it is 1/20,000. Please let me know if you have any fu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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