Our Abpromise guarantee covers the use of ab3138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Electron Microscopy||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Do not perform antigen retrieval.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
This antibody precipitates the small nuclear RNAs U1, U2, U4, U5, and U6 providing direct evidence that the Sm antigen resides on all of these RNA-protein complexes.
|ICC/IF||1/250. (See Abreview). Fix with 3% PFA.|
|IP||Use at an assay dependent concentration.|
Smith Antigen was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Mouse monoclonal to Smith Antigen and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab3138.
Secondary: Protein G-HRP at 1/500 dilution.
Band: 28 and 31kDa: Smith Antigen.