Overview

  • Product nameAnti-SMNDC1 antibody
    See all SMNDC1 primary antibodies
  • Description
    Mouse polyclonal to SMNDC1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length Human SMNDC1 (NP_005862.1).

  • Positive control
    • Human placenta lysate, PC-12 cell lysate, Raw 264.7 cell lysate, A-431 cell lysate, NIH/3T3 cell lysate, SMNDC1 transfected 293T cell lysate, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab89131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionNecessary for spliceosome assembly. Overexpression causes apoptosis.
  • Tissue specificityDetected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas.
  • Sequence similaritiesBelongs to the SMN family.
    Contains 1 Tudor domain.
  • Cellular localizationNucleus speckle. Nucleus > Cajal body. Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • 30 kDa splicing factor SMNrp antibody
    • MGC106917 antibody
    • MGC112663 antibody
    • SMN related protein antibody
    • SMN-related protein antibody
    • smndc1 antibody
    • SMNR antibody
    • SPF30 antibody
    • SPF30_HUMAN antibody
    • Splicing factor 30, survival of motor neuron-related antibody
    • Survival motor neuron domain containing 1 antibody
    • Survival motor neuron domain containing protein 1 antibody
    • Survival motor neuron domain-containing protein 1 antibody
    • Survival of motor neuron related splicing factor 30 antibody
    • Survival of motor neuron-related-splicing factor 30 antibody
    see all

Anti-SMNDC1 antibody images

  • Anti-SMNDC1 antibody (ab89131) at 1 µg/ml + Human placenta lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 27 kDa
  • Anti-SMNDC1 antibody (ab89131) at 1 µg/ml + PC-12 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 27 kDa
  • Anti-SMNDC1 antibody (ab89131) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 27 kDa
  • Anti-SMNDC1 antibody (ab89131) at 1 µg/ml + A-431 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 27 kDa
  • Anti-SMNDC1 antibody (ab89131) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg
    Developed using the ECL technique

    Predicted band size : 27 kDa
  • All lanes : Anti-SMNDC1 antibody (ab89131) at 1 µg/ml

    Lane 1 : SMNDC1 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Developed using the ECL technique

    Predicted band size : 27 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • ab89131 at 10 µg/ml staining SMNDC1 in HeLa cells by Immunofluorescence.

References for Anti-SMNDC1 antibody (ab89131)

ab89131 has not yet been referenced specifically in any publications.

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