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Our Abpromise guarantee covers the use of ab683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. PubMed: 21103378|
|IHC-P||1/400. For paraffin embedded sections, Carnoy's fixative may give better results than formalin.|
ab683 at 1/400 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue sections were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa Fluor® 594 conjugated goat anti-mouse IgG1 was used as the secondary.
ab683 staining smooth muscle Myosin heavy chain 11 in rat cells from cells from mesenteric artery by ICC/IF. Cells were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 2% serum for 30 minutes at 20°C. The primary antibody was diluted 1/300 and incubated with the sample for 14 hours at 4°C. Alexa Fluor® 488 chicken polyclonal to mouse Ig, diluted 1/400, was used as the secondary. Nuclei stained with Hoechst 33342.
ab683 staining smooth muscle Myosin heavy chain 11 in human cerebral artery tissue sections by Immunohistochemistry (IHC-P - Formalin/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton-X and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate. Samples were incubated with the primary antibody (1/500) for 16 hours at 4°C. A biotin-conjugated rabbit anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
ab683 staining smooth muscle Myosin heavy chain in human freshly isolated arterial myocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 30 minutes at 20°C. Samples were incubated with the primary antibody (1/300 in PBS) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated chicken anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.
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