Anti-smooth muscle Myosin heavy chain 11 antibody [1G12] (ab683)

Overview

  • Product name
    Anti-smooth muscle Myosin heavy chain 11 antibody [1G12]
    See all smooth muscle Myosin heavy chain 11 primary antibodies
  • Description
    Mouse monoclonal [1G12] to smooth muscle Myosin heavy chain 11
  • Specificity
    This antibody reacts with human, rabbit, and rat smooth muscle myosin heavy chain 11 (SM2). Does not cross-react with smooth muscle Myosin heavy chain 1.
  • Tested applications
    Suitable for: WB, IHC-Fr, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human smooth muscle Myosin heavy chain 11. The immunogen sequence exists in isoforms 3 and 4 but not isoforms 1 and 2 of the UniProt record P35749.
    Sequence:

    RGPPPQETSQ


    Database link: P35749-3

Properties

Applications

Our Abpromise guarantee covers the use of ab683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. PubMed: 21103378
IHC-Fr 1/400.
ICC/IF 1/250.
IHC-P 1/400. For paraffin embedded sections, Carnoy's fixative may give better results than formalin.

Target

  • Function
    Muscle contraction.
  • Tissue specificity
    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • Involvement in disease
    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • Sequence similarities
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • Domain
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • Cellular localization
    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH 11 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
    • Smooth muscle myosin heavy chain isoform SM2 antibody
    see all

Images

  • ab683 at 1/400 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue sections were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa Fluor® 594 conjugated goat anti-mouse IgG1 was used as the secondary.

    See Abreview

  • ab683 staining smooth muscle Myosin heavy chain 11 in rat cells from cells from mesenteric artery by ICC/IF. Cells were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 2% serum for 30 minutes at 20°C. The primary antibody was diluted 1/300 and incubated with the sample for 14 hours at 4°C. Alexa Fluor® 488 chicken polyclonal to mouse Ig, diluted 1/400, was used as the secondary. Nuclei stained with Hoechst 33342.

    See Abreview

  • ab683 staining smooth muscle Myosin heavy chain 11 in human cerebral artery tissue sections by Immunohistochemistry (IHC-P - Formalin/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton-X and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate. Samples were incubated with the primary antibody (1/500) for 16 hours at 4°C. A biotin-conjugated rabbit anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab683 staining smooth muscle Myosin heavy chain in human freshly isolated arterial myocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 30 minutes at 20°C. Samples were incubated with the primary antibody (1/300 in PBS) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated chicken anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Xu JG  et al. TGF-ß1-induced differentiation of SHED into functional smooth muscle cells. Stem Cell Res Ther 8:10 (2017). Read more (PubMed: 28114966) »
  • Christoforou N  et al. Core Transcription Factors, MicroRNAs, and Small Molecules Drive Transdifferentiation of Human Fibroblasts Towards The Cardiac Cell Lineage. Sci Rep 7:40285 (2017). Read more (PubMed: 28071742) »

See all 23 Publications for this product

Customer reviews and Q&As

Thank you for contacting Abcam.

Please find links below to a few antibodies that we have that recognize smooth muscle heavy chain 1 and 2:

http://www.abcam.com/Myosin-smooth-muscle-heavy-chain-1-and-2-antibody-EPR5335-ab124679.html ...

Read More

Thank you for contacting us.

The antibody ab683 will recognize SM2A and SM2B:

Human SM2A

http://www.ncbi.nlm.nih.gov/protein/13124875?report=genbank&log$=prottop&blast_rank=8&RID=R93GH1VT016

Human SM2B
...

Read More

Thank you for contacting us.

Yes, I'd be happy to replace ab683 with ab53219 (which is also an Anti-smooth muscle Myosin heavy chain 11 antibody). Since this antibody has not been tested in human samples, we would not be able to guarantee it...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Carotid Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)
Permeabilization
Yes - 0.1% Triton X-100 for 15 mins
Specification
Carotid Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 20 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Temporal Artery Biopsy)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6.0
Permeabilization
No
Specification
Temporal Artery Biopsy
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

April Rempel

Verified customer

Submitted Apr 30 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Sample
Human Cell (Human freshly isolated arterial myocyte)
Specification
Human freshly isolated arterial myocyte
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 31 2015

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Sample
Mouse Cultured Cells (primary vascular smooth muscle cell)
Specification
primary vascular smooth muscle cell
Permeabilization
Yes - 0.25% Triton-X100
Fixative
Formaldehyde
Username

Mr. Mc Shen

Verified customer

Submitted Jan 29 2015



Les anticorps ab682 et ab683 ne sont pas purifié et donc la concentration n’a pas été déterminée. Ces anticorps sont des ascites et nous estimons les concentrations d’être entre 5-10 mg/ml.

Thank you for contacting us.

Please find attached protocol for ab7817.

The IHC-P protocol for ab683 can be found in publication;

http://ops.adminsite.com/admin/abcamshare/CRM/attach/2013/3/1/CCE4718082_img-301091703.pdf
<...

Read More

Thank you for contacting us.

Your credit note ID for the refund is xxx.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund,...

Read More

1-10 of 36 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up