Anti-smooth muscle Myosin heavy chain I antibody [3F8 ] (ab682)

Overview

  • Product nameAnti-smooth muscle Myosin heavy chain I antibody [3F8 ]
    See all smooth muscle Myosin heavy chain I primary antibodies
  • Description
    Mouse monoclonal [3F8 ] to smooth muscle Myosin heavy chain I
  • SpecificityThis antibody reacts with smooth muscle myosin heavy chain 1 (SM1). Does not cross-react with smooth muscle myosin heavy chain 2.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    DADFNGTKASE

    , corresponding to C terminal amino acids 1962-1972 of smooth muscle Myosin heavy chain I

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferAscitic fluid
  • Concentration information loading...
  • PurityAscites
  • ClonalityMonoclonal
  • Clone number3F8
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/3000. Carnoy's fixative may give better results than formalin.
IHC-Fr 1/3000.
Flow Cyt Use at an assay dependent concentration. PubMed: 20618694
WB Use at an assay dependent concentration.

Target

  • RelevanceMYH11 is a smooth muscle myosin belonging to the myosin heavy chain family. It is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light chain subunits. It functions as a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ATP.
  • Database links
  • Alternative names
    • FAA4 antibody
    • MYH11 antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • SMHC antibody
    • SMMHC antibody
    see all

Anti-smooth muscle Myosin heavy chain I antibody [3F8 ] images

  • All lanes : Anti-smooth muscle Myosin heavy chain I antibody [3F8 ] (ab682) at 1/250 dilution

    Lane 1 : 30ug/lane human 293 cells (whole cell lysate) transiently transfected with a CBFB-SMMHC plasmid
    Lane 2 : 30ug/lane human 293 cells (whole cell lysate) transiently transfected with a CBFB-SMMHC plasmid

    Secondary
    Alkaline Phosphatase conjugated goat anti-mouse antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 68 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-smooth muscle Myosin heavy chain I antibody [3F8 ] (ab682)

This product has been referenced in:
  • van Dijk RA  et al. The natural history of aortic atherosclerosis: a systematic histopathological evaluation of the peri-renal region. Atherosclerosis 210:100-6 (2010). Read more (PubMed: 20031134) »
  • Maier CL  et al. Explant outgrowth, propagation and characterization of human pericytes. Microcirculation 17:367-80 (2010). Flow Cyt ; Human . Read more (PubMed: 20618694) »

See all 5 Publications for this product

Product Wall



Les anticorps ab682 et ab683 ne sont pas purifié et donc la concentration n’a pas été déterminée. Ces anticorps sont des ascites et nous estimons les concentrations d’être entre 5-10 mg/ml.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse lung)
Specification Mouse lung
Fixative Formaldehyde
Antigen retrieval step Enzymatic
Blocking step MOM Kit -Vector Labs as blocking agent for 1 hour(s) and 0 minute(s)
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Abcam user community

Verified customer

Submitted Nov 09 2006

I am pleased that you have been able to apply these antibodies and have some valuable feedback information to provide us with. With regards the antibodies that I recommended, ab682 and ab683 were the best characterized with the most positive customer f...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293 cells)
Loading amount 30 µg
Specification 293 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Aug 16 2006

Thank you for your enquiry. Both antibodies were obtained by immunizing synthesized C-terminal peptides specific for each sequence. Therefore, they do not cross-react each other. This is confirmed by immunoblot analysis and reported in the reference...

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Thank you for your enquiry. This antibody was generated by immunizing the synthetic peptide specific for C-terminal end of SM1. Therefore it does not cross-react with other isoforms; as reported by Aikawa et al. Circ Res 1993;73:1000-1012. More...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"