Anti-Smoothelin antibody (ab74379)

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Customer reviews and FAQs

Overview

  • Product name
    Anti-Smoothelin antibody
    See all Smoothelin primary antibodies
  • Description
    Rabbit polyclonal to Smoothelin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Smoothelin.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab74378.)

  • Positive control
    • This antibody gave a positive signal in the following Human lysates: Liver Tissue, Jurkat Whole Cell, SK N SH Whole Cell, Brain Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab74379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 95, 100 kDa (predicted molecular weight: 100 kDa).Can be blocked with Human Smoothelin peptide (ab74378).

Target

Anti-Smoothelin antibody images

  • Western blot - Anti-Smoothelin antibody (ab74379)
    Western blot - Anti-Smoothelin antibody (ab74379)
    All lanes : Anti-Smoothelin antibody (ab74379) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 100 kDa
    Observed band size : 95,100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,45 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References for Anti-Smoothelin antibody (ab74379)

ab74379 has not yet been referenced specifically in any publications.

Publishing research using ab74379? Please let us know so that we can cite the reference in this datasheet.

Product Wall

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3 Abreviews or Q&A

10
Votes: Highest First

Dear sir or madam, I am looking for a Smoothelin antibody for western blotting. I am interested in purchasing your ab74379 antibody for this purpose, but I can see in the product datasheet that it is not confirmed to react with mouse. Would it be possi...

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Thank you for your interest in our products.

Discount code: XXXXX

Expiration date: 02-March-2013

I am very pleased to hear you would like to test ab74379 with Mouse species. This code will give you 370€ off your next o...

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Dear xxxx,

Thank you very much for your assistance.Iwould like to order theab74379 antibody, could you please issue me with a testing discount code.

Regards

DISCOUNT CODE: xxxxxxx
Expiration date: 19th of August 2012

I am very pleased to hear you would like to accept our offer and test ab74379 inICC with mouse samples. This code will give you: 1 freeprimary antibodybefore the expiration date...

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Phone call requesting information on which antibody would be most suitable to use in ICC of mouse samples detecting Smoothelin between ab69724 or ab74379.

Thank you for contacting us in regards to using anti-Smoothelin antibody (ab69724 or ab74379) for ICC of mouse samples.

As discussed over the phone, unfortunately, whilst ab69724 has not been used with mouse samples as yet, ab74379 has only b...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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