Full length protein (Human smoothelin).
Our Abpromise guarantee covers the use of ab21108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/100 - 1/200.|
|IHC-P||1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||Use at an assay dependent concentration.|
|WB||1/100 - 1/200. Predicted molecular weight: 102 kDa.|
Paraffin-embedded human smooth muscle tissue stained for Smoothelin using ab21108 at 1/100 dilution in immunohistochemical analysis.
Frozen section of chicken gizzard striated muscle tissue stained for Smoothelin using ab21108 at 1/100 dilution in immunohistochemical analysis.
ab21108 staining human small intestine tissue sections by IHC-P. The sample was formaldehyde fixed and subjected to a heat mediated antigen retrieval in EDTA prior to incubation with ab21108 (1/1600) for 16 minutes at 25°C. Polymer detection was with DAB (no enhancement) and a hematoxylin counterstain.