Smoothelin antibody [R4A] (ab8969)

Product Name 

Smoothelin antibody [R4A]

Product type 

Primary antibodies

Description 

Mouse monoclonal [R4A] to Smoothelin

Immunogen 

Cytoskeletal extract of chicken gizzard.

Reacts with 

Hu, Cat, Chk, Dog, Mk, Pig

Does not react with 
(species key)

Ms, Rat, Hm

Specificity 

This antibody reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells.

Tested applications 
(see key)

ICC/IF, IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
ICC: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
For staining paraffin-embedded tissues pretreatment in 10 mM citrate buffer (pH 6.0) and heating for 3 times 5 minutes in a microwave is required.
Recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
IHC-Fr: Use at an assay dependent dilution.
WB: 1/100 - 1/1000.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic

Research areas 

Developmental Biology >> Organogenesis >> Skeletal development >> Muscle
Cardiovascular >> Vasculature >> Smooth muscle cell (SMC) >> Cytoskeleton
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class III >> Smoothelins

Relevance 

Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin.
Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two major isoforms of smoothelin have been reported, a 59-kDa smoothelin A in visceral smooth muscle cells and a 110-kDa smoothelin B in vascular smooth muscle cells. Both gene products are encoded by a single copy gene which is located on chromosome 22 and regulated by a dual promoter system in a tissue specific manner. The variation in the smoothelin proteins is achieved by alternative splicing in the calponin homology domain. The distribution of smoothelin positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Antibodies to smoothelin are powerful tools in evaluation of smooth muscle cell differentiation as occurs in atherosclerotic lesions.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

R4A

Myeloma 

Sp2/0-Ag14

Isotype 

IgG1

Purity 

Protein G purified

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Smoothelin antibody [R4A] images, references, products related to ab8969 and other tools.

Smoothelin antibody [R4A] images:

References for Smoothelin antibody [R4A] (ab8969)

  This product has been used in: (two most recent references)

Hu JJ et al. Time courses of growth and remodeling of porcine aortic media during hypertension: a quantitative immunohistochemical examination. J Histochem Cytochem 56:359-70 (2008). PubMed: 18071063

Nolazco G et al. Effect of muscle-derived stem cells on the restoration of corpora cavernosa smooth muscle and erectile function in the aged rat. BJU Int 101:1156-64 (2008). PubMed: 18294308

See all 10 publication references for this product.


If you publish research using ab8969 please let us know so that we can cite the reference on this datasheet.

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