Overview

  • Product nameAnti-Smoothened antibody
    See all Smoothened primary antibodies
  • Description
    Rabbit polyclonal to Smoothened
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide against N terminal of Human Smoothened conjugated to KLH.

  • Positive control
    • Human Liver & lung tissue, HuvEc cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 86 kDa.
ELISA 1/10000.
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionG protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.
  • Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family.
    Contains 1 FZ (frizzled) domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled family member 11 antibody
    • FZD11 antibody
    • Gx antibody
    • Gx protein antibody
    • Protein Gx antibody
    • SMO antibody
    • SMO_HUMAN antibody
    • SMOH antibody
    • Smoothened frizzled family receptor antibody
    • Smoothened homolog (Drosophila) antibody
    • Smoothened homolog antibody
    • Smoothened homolog precursor (SMO) (Gx protein) antibody
    • Smoothened seven transmembrane spanning receptor antibody
    see all

Anti-Smoothened antibody images

  • ab113438 at 10 µg/ml staining Smoothened in Human liver by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • ab113438 at 10 µg/ml staining Smoothened in Human lung by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • All lanes : Anti-Smoothened antibody (ab113438) at 1/500 dilution

    Lane 1 : Extracts from HuvEc cells
    Lane 2 : Extracts from HuvEc cells with Synthesized peptide


    Predicted band size : 86 kDa

References for Anti-Smoothened antibody (ab113438)

ab113438 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113438.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"