Anti-Smoothened antibody (ab209833)
Key features and details
- Rabbit polyclonal to Smoothened
- Suitable for: WB
- Reacts with: Zebrafish
- Isotype: IgG
Overview
-
Product name
Anti-Smoothened antibody
See all Smoothened primary antibodies -
Description
Rabbit polyclonal to Smoothened -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Recombinant fragment within Zebrafish Smoothened aa 471-686. The exact sequence is proprietary.
Database link: 30225 -
Positive control
- WB: zebrafish brain, zebrafish heart, zebrafish liver and zebrafish muscle extracts.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000.
|
Notes |
---|
WB
1/500 - 1/3000. |
Target
-
Function
G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia. -
Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 30225 Zebrafish
-
Alternative names
- Frizzled family member 11 antibody
- FZD11 antibody
- Gx antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab209833 has not yet been referenced specifically in any publications.