Overview

  • Product nameAnti-Smoothened antibody
    See all Smoothened primary antibodies
  • Description
    Goat polyclonal to Smoothened
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Dog, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-QSDDEPKRIKKS

    , corresponding to internal sequence amino acids 555-566 of Human Smoothened

  • Positive control
    • Human Bone Marrow lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab58591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 86 kDa). The observed molecular weight corresponds to findings with ab58574, a product of different design reacting to a different part of the same protein.

Target

Anti-Smoothened antibody images

  • Lane 1 : Anti-Smoothened antibody (ab58574) at 0.3 µg/ml (incubated for 1 hour)
    Lane 2 : Anti-Smoothened antibody (ab58591) at 0.3 µg/ml (incubated for 1 hour)

    Lane 1 : Human Bone Marrow lysate (35µg protein in RIPA buffer)
    Lane 2 : Human Bone Marrow lysate (35µg protein in RIPA buffer)

    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References for Anti-Smoothened antibody (ab58591)

ab58591 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"