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Synthetic peptide within Human Smoothened aa 190-240. The exact sequence is proprietary. The immunogen sequence is 100% homologous in human ( isoforms CRA_a, CRA_b, CRA_c), rhesus monkey and chimpanzee, 94% in horse, cow and dog, 88% in rat, mouse, chicken, opossum, zebrafish and Xenopus.
Our Abpromise guarantee covers the use of ab72130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 24136221
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.