This antibody gave a positive signal against Human Smoothened Partial Recombinant Protein (Tagged)
General notesab80683 is raised using the same immunogen as ab38686. Batches sold under ab80683 have passed QC testing in WB (Recombinant only). In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
FunctionG protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.
Sequence similaritiesBelongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain.
ICC/IF image of ab80683 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80683, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Smoothened antibody (ab80683)
has not yet been referenced specifically in any publications.
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