Overview

  • Product nameAnti-SMRC2 antibody
    See all SMRC2 primary antibodies
  • Description
    Rabbit polyclonal to SMRC2
  • Specificityab64853 detects endogenous levels of total SMRC2 protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal region of human SMRC2.

  • Positive control
    • Extracts from HUVEC cells and COLO205 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 133 kDa).
ELISA 1/10000.

Target

  • FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the SMARCC family.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF 170 antibody
    • BAF170 antibody
    • BRG1 associated factor 170 antibody
    • BRG1-associated factor 170 antibody
    • Chromatin remodeling complex BAF170 subunit antibody
    • CRACC 2 antibody
    • CRACC2 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 170 antibody
    • Rsc 8 antibody
    • Rsc8 antibody
    • SMARCC 2 antibody
    • Smarcc2 antibody
    • SMRC2_HUMAN antibody
    • SWI/SNF complex 170 kDa subunit antibody
    • SWI/SNF complex subunit SMARCC2 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin c2 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 2 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2 antibody
    • SWI3 like protein antibody
    see all

Anti-SMRC2 antibody images

  • All lanes : Anti-SMRC2 antibody (ab64853) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from COLO205 cells
    Lane 3 : Extracts from COLO205 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 133 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)

References for Anti-SMRC2 antibody (ab64853)

ab64853 has not yet been referenced specifically in any publications.

Product Wall

Gracias por contactarnos.

He chequeado la homología de dichos anticuerpos con BAF155, y el resultado es el siguiente:

ab64853: Al llevar a cabo un alineamiento de secuencias con BAFF15 (http://www.uniprot.org/uniprot/Q92922) ...

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Thank you for contacting us.  Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of ab64853. The immunogen sequence range for the antigen is com...

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