Overview

  • Product nameAnti-SMRC2 antibody
    See all SMRC2 primary antibodies
  • Description
    Rabbit polyclonal to SMRC2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human SMRC2.

    (Peptide available as ab71906.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Raji SK N SH

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 133 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionInvolved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the SMARCC family.
    Contains 1 SANT domain.
    Contains 1 SWIRM domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BAF 170 antibody
    • BAF170 antibody
    • BRG1 associated factor 170 antibody
    • BRG1-associated factor 170 antibody
    • Chromatin remodeling complex BAF170 subunit antibody
    • CRACC 2 antibody
    • CRACC2 antibody
    • Mammalian chromatin remodeling complex BRG1 associated factor 170 antibody
    • Rsc 8 antibody
    • Rsc8 antibody
    • SMARCC 2 antibody
    • Smarcc2 antibody
    • SMRC2_HUMAN antibody
    • SWI/SNF complex 170 kDa subunit antibody
    • SWI/SNF complex subunit SMARCC2 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin c2 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 2 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2 antibody
    • SWI3 like protein antibody
    see all

Anti-SMRC2 antibody images

  • All lanes : Anti-SMRC2 antibody (ab71907) at 1 µg/ml

    Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 2 : SK N SH (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 133 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 62 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab71907 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71907, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 cells at 5µg/ml.

References for Anti-SMRC2 antibody (ab71907)

ab71907 has not yet been referenced specifically in any publications.

Product Wall

Gracias por contactarnos.

He chequeado la homología de dichos anticuerpos con BAF155, y el resultado es el siguiente:

ab64853: Al llevar a cabo un alineamiento de secuencias con BAFF15 (http://www.uniprot.org/uniprot/Q92922) ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"