• Product nameAnti-Smt3 antibody
    See all Smt3 primary antibodies
  • Description
    Rabbit polyclonal to Smt3
  • Specificityab14405 recognizes SUMO-GFP fusion proteins and purified Smt3. It is predicted to bind to yeast SUMO (SMT) bound to other proteins. Cross reactivity with other homologs may occur but has not been specifically tested.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant protein (S. cerevisiae).

  • Positive control
    • Most yeast cell lysates can be used as a positive control without induction or stimulation.
  • General notesCentrifuge product if not completely clear after standing at room temperature.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit serum.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab14405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 12 kDa.
Note: Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.
ELISA 1/1000 - 1/5000.


  • FunctionNot known; suppressor of MIF2 mutations.
  • Sequence similaritiesBelongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Information by UniProt
  • Database links
    • Alternative names
      • DmSUMO 1 antibody
      • Small Ubiquitin-like modifier antibody
      • SMT3 antibody
      • SMT3_YEAST antibody
      • Ubiquitin like protein of the SUMO family antibody
      • Ubiquitin like protein SMT3 antibody
      • Ubiquitin-like protein SMT3 antibody
      see all

    Anti-Smt3 antibody images

    • All lanes : Anti-Smt3 antibody (ab14405) at 1/1000 dilution

      Lane 1 : 3 : 5 : 7 : 9 : 11 : 13, Lysate prepared from E.coli expressed Yeast SUMO protein and SUMO-GFP fusion protein after incubation with lysed insect cells
      Lane 2 : 4 : 6 : 8 : 10 : 12 : 14, Lysate prepared from SUMO-GFP fusion protein after incubation with lysed insect cells in the presence of 2% SDS

      Lysates/proteins at 2 µg per lane.
    • ab14405 detecting Smt3 in Escherichia coli recombinant protein (BL21(DE3) by Direct ELISA. Wells were coated with 10µg each of diluted control protein (without SUMO fusion - Negative Controls) and target protein (with SUMO fusion protein) in bicarbonate buffer. Wells were blocked with 5% milk for 1 hour at room temperature. The samples were incubated with primary antibody (1/5000 in TBST +5% milk) for 45 minutes. An HRP-conjugated goat polyclonal to rabbit IgG (1/40000) was used as the secondary antibody.

      See Abreview

    References for Anti-Smt3 antibody (ab14405)

    This product has been referenced in:
    • Lescasse R  et al. End-joining inhibition at telomeres requires the translocase and polySUMO-dependent ubiquitin ligase Uls1. EMBO J 32:805-15 (2013). Read more (PubMed: 23417015) »
    • Khan MA  et al. Knockout of caspase-like gene, YCA1, abrogates apoptosis and elevates oxidized proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 102:17326-31 (2005). WB ; Saccharomyces cerevisiae . Read more (PubMed: 16301538) »

    See all 2 Publications for this product

    Product Wall

    The immunogen is full-length protein, untagged, and the amino acid sequence is given at the UniProt entry for S. cerevisiae Smt3,

    http://www.uniprot.org/uniprot/Q12306 .

    This antibody has not been tested by immunprecipitation, or ...

    Read More
    Application Western blot
    Sample Saccharomyces cerevisiae Cell lysate - whole cell (log phase yeast cells)
    Loading amount 100 µg
    Specification log phase yeast cells
    Gel Running Conditions Reduced Denaturing (6-12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C

    Dr. O Kerscher

    Verified customer

    Submitted Nov 10 2011

    Application ELISA
    Sample Escherichia coli Recombinant protein (BL21(DE3))
    Specification BL21(DE3)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Type Direct

    Abcam user community

    Verified customer

    Submitted May 28 2010

    Application Western blot
    Sample Escherichia coli Recombinant protein (BL21(DE3))
    Loading amount 0.5 µg
    Specification BL21(DE3)
    Gel Running Conditions Reduced Denaturing (4-20%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Apr 26 2010

    Thank you for your enquiry. I am pleased that you are happy with the performance of Smt3 antibody (ab14405). Please can I encourage you to leave an Abreview. This can be done by clicking on the "Abreview" tab on the product datasheet. In return we ...

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    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Please rest ...

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    Thank you for your enquiry. Ab5211 is a Fast-Track antibody and has not yet been fully characterized, therefore we cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide. We do have some prelimin...

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