Overview

  • Product nameAnti-SMUG1 antibody
    See all SMUG1 primary antibodies
  • Description
    Rabbit polyclonal to SMUG1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 110-159 of Human SMUG1 (IVGPVLTPPQ EHPKRPVLGL ECPQSEVSGA RFWGFFRNLC GQPEVFFHHC).

  • Positive control
    • HeLa cell lysates

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab104663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Suggested dilution in 5% skim milk / PBS buffer Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionRecognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FDG antibody
    • HMUDG antibody
    • MGC104370 antibody
    • Single strand selective monofunctional uracil DNA glycosylase 1 antibody
    • Single strand selective monofunctional uracil DNA glycosylase antibody
    • Single-strand selective monofunctional uracil DNA glycosylase antibody
    • SMUG 1 antibody
    • Smug1 antibody
    • SMUG1 protein antibody
    • SMUG1_HUMAN antibody
    • UNG 3 antibody
    • UNG3 antibody
    see all

Anti-SMUG1 antibody images

  • Anti-SMUG1 antibody (ab104663) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa

References for Anti-SMUG1 antibody (ab104663)

ab104663 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepDES19 cells)
Loading amount 100000 cells
Specification HepDES19 cells
Treatment pcDNA3.1 or pcDNA-SMUG1 transfected
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Shijian Zhang

Verified customer

Submitted Oct 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"