Overview

  • Product nameAnti-SMUG1 antibody
    See all SMUG1 primary antibodies
  • Description
    Goat polyclonal to SMUG1
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    PQAFLLGSIHEPA-C

    , corresponding to N terminal amino acids 2-14 of Human SMUG1.

  • Positive control
    • Human brain cortex tissue; MOLT-4 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 29 kDa.
ELISA 1/32000.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRecognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • FDG antibody
    • HMUDG antibody
    • MGC104370 antibody
    • Single strand selective monofunctional uracil DNA glycosylase 1 antibody
    • Single strand selective monofunctional uracil DNA glycosylase antibody
    • Single-strand selective monofunctional uracil DNA glycosylase antibody
    • SMUG 1 antibody
    • Smug1 antibody
    • SMUG1 protein antibody
    • SMUG1_HUMAN antibody
    • UNG 3 antibody
    • UNG3 antibody
    see all

Anti-SMUG1 antibody images

  • Human Brain Cortex tissue (formalin-fixed, paraffin-embedded) stained with ab115572 at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-SMUG1 antibody (ab115572) at 0.5 µg/ml (1 hour incubation) + MOLT-4 cell lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 29 kDa

References for Anti-SMUG1 antibody (ab115572)

ab115572 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"