Recombinant Anti-SMUG1 antibody [EPR15624] (ab192240)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15624] to SMUG1
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SMUG1 antibody [EPR15624]
See all SMUG1 primary antibodies -
Description
Rabbit monoclonal [EPR15624] to SMUG1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HAP1, 293 and MOLT4 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15624 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192240 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/100.
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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Notes |
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IP
1/100. |
WB
1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23583 Human
- Omim: 607753 Human
- SwissProt: Q53HV7 Human
- Unigene: 632721 Human
- Unigene: 731659 Human
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Alternative names
- FDG antibody
- HMUDG antibody
- MGC104370 antibody
see all
Images
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All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : SMUG1 knockout HAP1 cell lysate
Lane 3 : MOLT-4 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDaLanes 1 - 4: Merged signal (red and green). Green - ab192240 observed at 30 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab192240 was shown to react with SMUG1 in wild-type HAP1 cells in western blot with loss of signal observed in SMUG1 knockout sample. Wild-type and SMUG1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab192240 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab192240 at 1/100 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
All lanes : Anti-SMUG1 antibody [EPR15624] (ab192240) at 1/5000 dilution
Lane 1 : MOLT4 cell lysate
Lane 2 : 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 30 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab192240 has been referenced in 3 publications.
- Petljak M et al. Mechanisms of APOBEC3 mutagenesis in human cancer cells. Nature 607:799-807 (2022). PubMed: 35859169
- Hewitt G et al. Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD. Mol Cell 81:767-783.e11 (2021). PubMed: 33333017
- Kroustallaki P et al. SMUG1 Promotes Telomere Maintenance through Telomerase RNA Processing. Cell Rep 28:1690-1702.e10 (2019). PubMed: 31412240