Anti-SMURF 2 antibody (ab17919)

Overview

  • Product nameAnti-SMURF 2 antibody
    See all SMURF 2 primary antibodies
  • Description
    Rabbit polyclonal to SMURF 2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    DGWEERRTASGRIQ

    conjugated to KLH, corresponding to C terminal amino acids 161-174 of Human SMURF 2.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab17919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB 1/1000. Predicted molecular weight: 86 kDa. A nonspecific band is also observed at ~50kDa in HepG2 lysates.

Target

  • FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
  • Tissue specificityWidely expressed.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 3 WW domains.
  • DomainThe second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
    The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.
  • Post-translational
    modifications
    Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
  • Cellular localizationNucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • E3 ubiquitin-protein ligase SMURF2 antibody
    • EC 6.3.2. antibody
    • hSMURF2 antibody
    • MGC138150 antibody
    • Smad specific E3 ubiquitin ligase 2 antibody
    • SMAD specific E3 ubiquitin protein ligase 2 antibody
    • SMAD ubiquitination regulatory factor 2 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 2 antibody
    • SMUF2_HUMAN antibody
    • Smurf2 antibody
    • Ubiquitin protein ligase SMURF2 antibody
    see all

Anti-SMURF 2 antibody images

  • ab17919 staining SMURF 2 in Xenopus Laevis cultured retinal ganglion cell growth cone tissue by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 1 hour at 21°C. The primary antibody was diluted 1/250 in PBS and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/1000.

References for Anti-SMURF 2 antibody (ab17919)

ab17919 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (cultured retinal ganglion cell growth cone)
Specification cultured retinal ganglion cell growth cone
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% triton x-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

Submitted Nov 24 2010

Thank you for the details that you have provided. At this point I would suggest using positive controls with these antibodies. For ab17919, HepG2 lysate is recommended and for ab9984, we would suggest using a type of human lymphoid tissue as the highes...

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