• Product name
  • Description
    Rabbit polyclonal to SMURF 2
  • Host species
  • Specificity
    This antibody is specific for SMURF 2 (N term).
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide (10-30 aa in length) at the N-term of first 50 aa of human SMURF 2.

  • Positive control
    • Mouse brain tissue lysate and breast carcinoma tissue.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab38543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).


  • Function
    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
  • Tissue specificity
    Widely expressed.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 3 WW domains.
  • Domain
    The second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
    The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.
  • Post-translational
    Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
  • Cellular localization
    Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase SMURF2 antibody
    • EC 6.3.2. antibody
    • hSMURF2 antibody
    • MGC138150 antibody
    • Smad specific E3 ubiquitin ligase 2 antibody
    • SMAD specific E3 ubiquitin protein ligase 2 antibody
    • SMAD ubiquitination regulatory factor 2 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 2 antibody
    • SMUF2_HUMAN antibody
    • Smurf2 antibody
    • Ubiquitin protein ligase SMURF2 antibody
    see all


  • Anti-SMURF 2 antibody (ab38543) at 1/100 dilution + Mouse brain tissue lysate

    Predicted band size: 86 kDa
    Observed band size: 86 kDa

  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38543 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.


This product has been referenced in:
  • Itman C  et al. TGFß superfamily signaling regulators are differentially expressed in the developing and adult mouse testis. Spermatogenesis 1:63-72 (2011). Read more (PubMed: 21866277) »
  • Chen YL  et al. Smad6 inhibits the transcriptional activity of Tbx6 by mediating its degradation. J Biol Chem 284:23481-90 (2009). WB ; Mouse . Read more (PubMed: 19561075) »

See all 3 Publications for this product

Customer reviews and Q&As

Western blot
Human Cell lysate - whole cell (Berast Cancer)
Loading amount
100000 cells
Berast Cancer
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Ohad Tarcic

Verified customer

Submitted Dec 03 2012

Thank you for your Abreview. I am sorry that this antibody did not work for you.
In order to help you get a better result from this antibody I would like to like to give you a few suggestions.
1. I would suggest using a lysis buffer such as R...

Read More

Thank you for sharing that information.

Unfortunately xxxx is not in the office today but I will try to help as much as I can in this case. Having reviewed the protocol details supplied by the customer, there are a few suggestions I would mak...

Read More

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

Read More

Thank you for sending me this image. This data will be used to keep records of how our products are performing and for internal invetigation if needed. I noted that all tissue lysates that you have used have given the same non-specific signal. However ...

Read More

Thank you for taking time to send us your protocols. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring pro...

Read More

Thank you for contacting us. I would like to ask you if you might send me the protocols used with these productsso that we can gather further information regarding the samples tested and the protocol used. Once we receive your response, we will look at...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Mouse Cell (Oocyte)
Yes - 0.5% Triton-x
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C


Verified customer

Submitted Jun 20 2011


Sign up