Overview

  • Product nameAnti-SMURF1 antibody [1D7]
    See all SMURF1 primary antibodies
  • Description
    Mouse monoclonal [1D7] to SMURF1
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IF, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant Human SMURF1 protein (NP_851994.1).

  • Positive control
    • Human Prostate tissue; RAW 264.7 cell lysate; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab117552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 83 kDa.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Sandwich ELISA Use at an assay dependent concentration.

Target

  • FunctionE3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase SMURF1 antibody
    • hSMURF1 antibody
    • KIAA1625 antibody
    • Smad specific E3 ubiquitin ligase 1 antibody
    • SMAD specific E3 ubiquitin protein ligase 1 antibody
    • Smad ubiquitination regulatory factor 1 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 1 antibody
    • SMUF1_HUMAN antibody
    • SMURF 1 antibody
    • smurf1 antibody
    see all

Anti-SMURF1 antibody [1D7] images

  • ELISA of the detection limit for recombinant proprietary tagged SMURF1 is approximately 0.1ng/ml as a capture antibody (ab117552).
  • ab117552 at 10µg/ml dilution staining SMURF1 in HeLa cells by Immunofluorescence.
  • ab117552, at 5 µg/ml, staining SMURF1 in Formalin-fixed, Paraffin-embedded Human Prostate tissue by Immunohistochemistry.
  • Immunoprecipitation of SMURF1 transfected lysate using anti-SMURF1 monoclonal antibody (ab117552) and Protein A Magnetic Beads.
  • Anti-SMURF1 antibody [1D7] (ab117552) + RAW 264.7 cell lysate

    Predicted band size : 83 kDa
  • All lanes : Anti-SMURF1 antibody [1D7] (ab117552)

    Lane 1 : 293T lysate, transfected with SMURF1 protein
    Lane 2 : 293T lysate, mock transfected


    Predicted band size : 83 kDa

References for Anti-SMURF1 antibody [1D7] (ab117552)

This product has been referenced in:
  • Xie P  et al. The covalent modifier Nedd8 is critical for the activation of Smurf1 ubiquitin ligase in tumorigenesis. Nat Commun 5:3733 (2014). Read more (PubMed: 24821572) »

See 1 Publication for this product

Product Wall

As shown on the http://www.abcam.com/ab117552, ab117552has been tested in Mouse and in ICC/IF, it is therefore guaranteed to work in these particularspecies andapplication and we cannot offer a testing discount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"