Overview

  • Product nameAnti-SMURF1 antibody
    See all SMURF1 primary antibodies
  • Description
    Goat polyclonal to SMURF1
  • SpecificityThis antibody is expected to recognise both reported isoforms (NP_065162.1 and NP_851994.1).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity

    Predicted to work with: Human
  • Immunogen

    Synthetic peptide:

    PSEGSLEDEELPAQ

    , corresponding to Internal sequence amino acids 359-372 of Human SMURF1

  • Positive control
    • Whole cell lysate prepared from human lung carcinoma cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab40823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Can be blocked with SMURF1 peptide (359-372) (ab45831). See Abreview.

Target

  • FunctionE3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesContains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E3 ubiquitin-protein ligase SMURF1 antibody
    • hSMURF1 antibody
    • KIAA1625 antibody
    • Smad specific E3 ubiquitin ligase 1 antibody
    • SMAD specific E3 ubiquitin protein ligase 1 antibody
    • Smad ubiquitination regulatory factor 1 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 1 antibody
    • SMUF1_HUMAN antibody
    • SMURF 1 antibody
    • smurf1 antibody
    see all

References for Anti-SMURF1 antibody (ab40823)

ab40823 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (lung carcinoma cells)
Loading amount 20 µg
Specification lung carcinoma cells
Blocking step Odyssey block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100%
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Submitted Oct 22 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"