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Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
Function E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
Pathway Protein modification; protein ubiquitination.
Sequence similarities Contains 1 C2 domain. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. Contains 2 WW domains.
Post-translational modifications Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
Cellular localization Cytoplasm.
Information by UniProt
E3 ubiquitin-protein ligase SMURF1 antibody
Anti-SMURF1 antibody images
Western blot - SMURF1 antibody (ab94480)
All lanes : Anti-SMURF1 antibody (ab94480) at 1 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 3 : MG-63 (Human osteosarcoma cell line) Whole Cell Lysate Lane 4 : Human bone tumor tissue lysate - total protein ( ab29359) Lane 5 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution Developed using the ECL technique Performed under reducing conditions. Predicted band size : 86 kDa Observed band size : 86 kDa Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands. Exposure time : 12 minutes
References for Anti-SMURF1 antibody (ab94480)
has not yet been referenced specifically in any publications.
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