Anti-SMYD1 antibody (ab32482)

Overview

  • Product nameAnti-SMYD1 antibody
    See all SMYD1 primary antibodies
  • Description
    Rabbit polyclonal to SMYD1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human SMYD1.

    (Peptides available as ab34470, ab34471 and ab34470.)

  • Positive control
    • This antibody gave a positive result in the following lysates: Skeletal Muscle (Human) Tissue Lysate - adult normal tissue. Testis (Human) Tissue Lysate - adult normal tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab32482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

Anti-SMYD1 antibody images

  • All lanes : Anti-SMYD1 antibody (ab32482) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330) with Human SMYD1 peptide (ab34470) at 1 µg/ml
    Lane 4 : Human testis tissue lysate - total protein (ab30257) with Human SMYD1 peptide (ab34470) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

References for Anti-SMYD1 antibody (ab32482)

ab32482 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your interest in our SMYD1 antibodies, and for bringing the zebrafish article to our attention. Unfortunately, I do not see an order from any of the instituions listed in the author affilitaions section. We also have not removed any SMYD1...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse E9.5 Sagittal Section)
Specification Mouse E9.5 Sagittal Section
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization Yes - Tween20
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Dr. jesse mager

Verified customer

Submitted Jan 20 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Aug 20 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (heart)
Specification heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Permeabilization No
Blocking step Sequential peroxidase (5min) and protein (10min) block (prediluted) as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Aug 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"