Overview

  • Product nameAnti-SMYD1 antibody
    See all SMYD1 primary antibodies
  • Description
    Rabbit polyclonal to SMYD1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SMYD1.

    (Peptide available as ab34471.)

  • Positive control
    • This antibody gave a positive result in human Skeletal Muscle Tissue Lysate (adult normal tissue). IF/ICC: HeLa cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab34472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

Anti-SMYD1 antibody images

  • ICC/IF image of ab34472 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34472, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.



  • Predicted band size : 56 kDa

References for Anti-SMYD1 antibody (ab34472)

This product has been referenced in:
  • Just S  et al. The myosin-interacting protein SMYD1 is essential for sarcomere organization. J Cell Sci 124:3127-36 (2011). IHC ; Zebrafish . Read more (PubMed: 21852424) »
  • Sirinupong N  et al. Crystal Structure of Cardiac-specific Histone Methyltransferase SmyD1 Reveals Unusual Active Site Architecture. J Biol Chem 285:40635-44 (2010). WB . Read more (PubMed: 20943667) »

See all 2 Publications for this product

Product Wall

Thank you for your interest in our SMYD1 antibodies, and for bringing the zebrafish article to our attention. Unfortunately, I do not see an order from any of the instituions listed in the author affilitaions section. We also have not removed any SMYD1...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"