Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
Belongs to the histone-lysine methyltransferase family. Contains 1 MYND-type zinc finger. Contains 1 SET domain.
Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
Confocal immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SMYD3 with ab155018 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody at 1/2000 dilution (red).
ab155018 immunoprecipitates SMYD3 protein in IP experiments.
IP Sample: 1000 µg HeLa whole cell lysate Lane 1. Control with 2.5 µg of preimmune rabbit IgG Lane 2. Immunoprecipitation of SMYD3 protein using 2.5 µg of ab155018. The immunoprecipitated SMYD3 protein was detected using ab155018 at 1/1000 dilution. 12% SDS-PAGE.