Recombinant
RabMAb

Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498)

Overview

  • Product name
    Anti-SMYD3 antibody [EPR11106(2)] - N-terminal
    See all SMYD3 primary antibodies
  • Description
    Rabbit monoclonal [EPR11106(2)] to SMYD3 - N-terminal
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SMYD3 aa 1-100. The exact sequence is proprietary.
    Database link: Q9H7B4

  • Positive control
    • HeLa, 293, MCF-7 and T47-D cell lysates, Human testis and skeletal muscle tissue, HeLa cells, 293 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab183498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
ICC/IF 1/500.
IP 1/40 - 1/60.

Target

  • Function
    Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.
  • Tissue specificity
    Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.
  • Sequence similarities
    Belongs to the histone-lysine methyltransferase family.
    Contains 1 MYND-type zinc finger.
    Contains 1 SET domain.
  • Cellular localization
    Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA74P14.1 (novel protein) antibody
    • bA74P14.1 antibody
    • FLJ21080 antibody
    • histone lysine N methyltransferase SMYD3 antibody
    • KMT3E antibody
    • MGC104324 antibody
    • SET and MYND domain containing 3 antibody
    • SET and MYND domain containing protein 3 antibody
    • SET and MYND domain-containing protein 3 antibody
    • SMYD 3 antibody
    • Smyd3 antibody
    • SMYD3 protein antibody
    • SMYD3_HUMAN antibody
    • Zinc finger MYND domain containing 1 antibody
    • Zinc finger MYND domain containing protein 1 antibody
    • Zinc finger MYND domain-containing protein 1 antibody
    • Zinc finger protein subfamily 3A MYND domain containing 1 antibody
    • Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody
    • ZMYND 1 antibody
    • ZMYND1 antibody
    • ZNFN3A1 antibody
    see all

Images

  • All lanes : Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293 lysate
    Lane 3 : T47-D lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 49 kDa
  • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling SMYD3 at 1/100 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

  • Flow cytometric analysis of HeLa cells fixed in 20 °C acetone labeling SMYD3 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 (red). Counterstained with DAPI (blue)

  • Immunoprecipitation of HeLa cells labeling SMYD3 with ab183498 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.  

  • Flow cytometric analysis of 293 cells fixed in 2% paraformaldehyde labeling SMYD3 with ab183498 at 1/110 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit Monoclonal IgG was used as an isotype control.

  • Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/2000 dilution + MCF-7 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 49 kDa
  • Immunohistochemical analysis of paraffin-embedded Human testis labeling SMYD3 with ab183498 at 1/100 dilution and  HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

References

ab183498 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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