• Product nameAnti-SNAIL antibody
    See all SNAIL primary antibodies
  • Description
    Goat polyclonal to SNAIL
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 8-19 of Human SNAIL

  • Positive control
    • This antibody gave a positive signal when tested against the SNAIL recombinant protein.



Our Abpromise guarantee covers the use of ab53519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 3 µg/ml.
IHC-P Use at an assay dependent dilution.


  • FunctionInvolved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
  • Tissue specificityExpressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
  • Sequence similaritiesBelongs to the snail C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • Post-translational
    Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
    Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
    O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
    ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.
  • Cellular localizationNucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Information by UniProt
  • Database links
  • Alternative names
    • dJ710H13.1 antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snail 1 homolog antibody
    • Snail 1 zinc finger protein antibody
    • SNAIL antibody
    • Snail homolog 1 (Drosophila) antibody
    • SNAIL, Drosophila, homolog of, 1 antibody
    • SNAIL1 antibody
    • Zinc finger protein SNAI1 antibody
    see all

Anti-SNAIL antibody images

  • All lanes : Anti-SNAIL antibody (ab53519) at 1/500 dilution

    Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
    Lane 2 : SLUG (SNAI2) Human Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    Exposure time : 12 minutes
    Ab53519 recognizes the tagged recombinant SNAIL protein which has an expected molecular weight of 68 kDa

References for Anti-SNAIL antibody (ab53519)

This product has been referenced in:
  • Park SH  et al. Itch E3 ubiquitin ligase positively regulates TGF-ß signaling to EMT via Smad7 ubiquitination. Mol Cells 38:20-5 (2015). WB ; Human . Read more (PubMed: 25518932) »
  • Zander MA  et al. The Snail transcription factor regulates the numbers of neural precursor cells and newborn neurons throughout mammalian life. PLoS One 9:e104767 (2014). IHC-P ; Mouse . Read more (PubMed: 25136812) »

See all 10 Publications for this product

Product Wall

Thank you for your patience while I investigated this with the lab.

They recommended not to incubate the primary overnight. They thought this may contribute tonon-specific background after overnight incubations. They recommend to incubate for...

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Thank you for your email. I apologize for the delay as I have to leave for a conference.

I have also received an answer from lab. They have suggested that the recombinant protein they have used is tagged with GST which is why the band size see...

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Thank you for contacting us.

I apologize for the delay in this reply as I was awaiting our Products Team to add the blocking peptide to our catalogue. We have added it as ab134217. We have to update the page to show the price before you can p...

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Thank you for contacting us.

Could you provide the following details

- Cell type e.g. Human hela cells lysates
- Provide image
- Blocking conditions for both antibodies
- Dilutions of both primary antibodies
- Informat...

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Expiration date: 28/04/2012

I am very pleased to hear you would like to accept our offer and testab53519 in WB and IHC on human samples. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before...

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Thank you for contacting us. We did not test the antibody for IHC but added the application to the datasheet based on a customer's feedback. Their protocol is contained in their review at the following link, and in the reference listed at the bottom...

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Thanks for contacting us with your question. The rabbit SNAIL antibody ab82846 was not produced with the same immunogen as ab85931. Ab82846 has been tested for cross-reactivity with recombinant SLUG in Western blot and there is no cross-reactivity (see...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (lung adenocarcinoma induced by urethane)
Specification lung adenocarcinoma induced by urethane
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

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Verified customer

Submitted Oct 21 2010