Anti-SNAIL + SLUG antibody (ab85936)
Key features and details
- Rabbit polyclonal to SNAIL + SLUG
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SNAIL + SLUG antibody
See all SNAIL + SLUG primary antibodies -
Description
Rabbit polyclonal to SNAIL + SLUG -
Host species
Rabbit -
Specificity
Replenishment batches of ab85936 are tested in WB. Previous batches were additionally validated in IHC-P. This application is still expected to work and is covered by our Abpromise guarantee.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: RatDoes not react with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human SNAIL Full-length Recombinant Protein (Tagged). IHC-P: Human breast ductal carcinoma.
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General notes
Batches sold under ab85936 have passed QC testing in Western Blot (Recombinant only) and IHC. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Please note this antibody has only been shown to detect recombinant protein and we are unable to detect endogenous protein by western blot, in our lab. Please contact our scientific support services if you have any queries regarding this antibody.
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IHC-P | (2) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa. Please note this antibody has only been shown to detect recombinant protein and we are unable to detect endogenous protein by western blot, in our lab. Please contact our scientific support services if you have any queries regarding this antibody. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Function: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. -
Cellular localization
Slug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. -
Database links
- Entrez Gene: 6591 Human
- Entrez Gene: 6615 Human
- Entrez Gene: 116490 Rat
- Entrez Gene: 25554 Rat
- Omim: 604238 Human
- SwissProt: O43623 Human
- SwissProt: O95863 Human
- SwissProt: O08954 Rat
see all -
Alternative names
- dJ710H13.1 antibody
- MGC10182 antibody
- Neural crest transcription factor Slug antibody
see all
Images
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IHC image of SNAIL + SLUG staining in a section of formalin-fixed paraffin-embedded normal human breast carcinoma* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab85936, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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All lanes : Anti-SNAIL + SLUG antibody (ab85936) at 1 µg/ml
Lane 1 : SLUG (SNAI2) Human - GST Tagged Recombinant Protein
Lane 2 : SNAIL (SNAI1) - GST Tagged Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Please note this antibody has only been shown to detect recombinant protein and we are unable to detect endogenous protein by western blot, in our lab. Please contact our scientific support services if you have any queries regarding this antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab85936 has been referenced in 33 publications.
- Guo Y et al. Metabolic-associated signature and hub genes associated with immune microenvironment and prognosis in bladder cancer. Mol Carcinog 62:185-199 (2023). PubMed: 36250643
- Zhou D et al. Musashi-1 and miR-147 Precursor Interaction Mediates Synergistic Oncogenicity Induced by Co-Infection of Two Avian Retroviruses. Cells 11:N/A (2022). PubMed: 36291177
- Tang S et al. PAFAH1B3 predicts poor prognosis and promotes progression in lung adenocarcinoma. BMC Cancer 22:525 (2022). PubMed: 35534807
- Chen S et al. Cancer-associated fibroblast-induced M2-polarized macrophages promote hepatocellular carcinoma progression via the plasminogen activator inhibitor-1 pathway. Int J Oncol 59:N/A (2021). PubMed: 34195849
- Dill TL et al. The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition. Development 148:N/A (2021). PubMed: 33298462