• Product nameAnti-SNAIL + SLUG antibody
    See all SNAIL + SLUG primary antibodies
  • Description
    Rabbit polyclonal to SNAIL + SLUG
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Recombinant Fragment
    Predicted to work with: RatDoes not react with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SNAIL.

    (Peptide available as ab19126.)

  • Positive control
    • This antibody gave a positive signal against Human SNAIL Full-length Recombinant Protein (Tagged)
  • General notesBatches sold under ab85936 have passed QC testing in Western Blot (Recombinant only) and IHC. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.



Our Abpromise guarantee covers the use of ab85936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 29 kDa.Can be blocked with Human SNAIL peptide (ab19126). Please note this antibody has only been shown to detect recombinant protein and we are unable to detect endogenous protein by western blot, in our lab. Please contact our scientific support services if you have any queries regarding this antibody.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • RelevanceFunction: SNAIL is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription. SLUG is a transcriptional repressor, involved in the generation and migration of neural crest cells. PTM: SNAIL is phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Similarity: Both SNAIL and SLUG belong to the snail C2H2-type zinc-finger protein family. Tissue specificity: SNAIL is expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. SLUG is expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle.
  • Cellular localizationSlug is generally nuclear, while Snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Database links
  • Alternative names
    • dJ710H13.1 antibody
    • MGC10182 antibody
    • Neural crest transcription factor Slug antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog 2 antibody
    • Protein snail homolog antibody
    • Slug homolog zinc finger protein antibody
    • Slug zinc finger protein antibody
    • SLUGH antibody
    • SLUGH 1 antibody
    • SLUGH1 antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI 2 antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snai2 antibody
    • SNAI2_HUMAN antibody
    • Snail 2 antibody
    • Snail homolog 1 (Drosophila) antibody
    • Snail homolog 2 antibody
    • Snail2 antibody
    • WS 2D antibody
    • WS2D antibody
    • Zinc finger protein SLUG antibody
    • Zinc finger protein SNAI1 antibody
    • Zinc finger protein SNAI2 antibody
    see all

Anti-SNAIL + SLUG antibody images

  • All lanes : Anti-SNAIL + SLUG antibody (ab85936) at 1 µg/ml

    Lane 1 : SLUG (SNAI2) Human - GST Tagged Recombinant Protein
    Lane 2 : SNAIL (SNAI1) - GST Tagged Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

    Exposure time : 30 seconds
  • IHC image of ab85936 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85936, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-SNAIL + SLUG antibody (ab85936)

This product has been referenced in:
  • Nishioka M  et al. Fibroblast-epithelial cell interactions drive epithelial-mesenchymal transition differently in cells from normal and COPD patients. Respir Res 16:72 (2015). IHC-P ; Human . Read more (PubMed: 26081431) »
  • Hanker AB  et al. Mutant PIK3CA accelerates HER2-driven transgenic mammary tumors and induces resistance to combinations of anti-HER2 therapies. Proc Natl Acad Sci U S A 110:14372-7 (2013). Read more (PubMed: 23940356) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (mammary gland tumors)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate antigen retrieval
Permeabilization No
Specification mammary gland tumors
Blocking step 10% goat serum in 1% BSA/PBS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 27°C
Fixative Formaldehyde

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Submitted Jan 19 2016