Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab3340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa.Can be blocked with SNAP23 peptide (ab4956). 23kDa band represents SNAP 23 from rat brain protein extract.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration. Used at a concentration of 2 ug/ml for 1 hr (see Abreview).

Target

  • FunctionEssential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
  • Tissue specificityUbiquitous. Highest levels where found in placenta.
  • Sequence similaritiesBelongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • Cellular localizationCell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HsT17016 antibody
    • LS-B8340 antibody
    • SNAP 23 antibody
    • SNAP-23 antibody
    • SNAP23 antibody
    • SNAP23A antibody
    • SNAP23B antibody
    • SNP23_HUMAN antibody
    • Synaptosomal associated protein 23 antibody
    • Synaptosomal associated protein 23kDa antibody
    • Synaptosomal associated protein antibody
    • Synaptosomal-associated protein 23 antibody
    • Vesicle membrane fusion protein SNAP 23 antibody
    • Vesicle membrane fusion protein SNAP23 antibody
    • Vesicle-membrane fusion protein SNAP-23 antibody
    see all

Anti-SNAP23 antibody images

  • Western blot detection of SNAP23 on rat brain protein extract using ab3340.

  • ab3340 (1ug/ml) staining SNAP23 in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cellular membrane compartments of the lymphatic nodules.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab3340 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

References for Anti-SNAP23 antibody (ab3340)

This product has been referenced in:
  • Williams KC & Coppolino MG SNARE-dependent interaction of Src, EGFR and ß1 integrin regulates invadopodia formation and tumor cell invasion. J Cell Sci 127:1712-25 (2014). WB . Read more (PubMed: 24496451) »
  • Williams KC  et al. SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate trafficking of membrane type 1-matrix metalloproteinase (MT1-MMP) during invadopodium formation and tumor cell invasion. Mol Biol Cell 25:2061-70 (2014). WB, IP, ICC/IF ; Human . Read more (PubMed: 24807903) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (COS7)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification COS7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Oct 20 2015

Application Western blot
Sample Dog Cell lysate - whole cell (Madin-Darby canine kidney (MDCK) cell)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification Madin-Darby canine kidney (MDCK) cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Oct 02 2015

Application Western blot
Sample Cow Cell lysate - whole cell (Bovine aortic endothelial cell)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification Bovine aortic endothelial cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 26 2015

Application Western blot
Sample Rat Cell lysate - whole cell (RBL-2H3)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification RBL-2H3
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 08 2015

Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification HEK293T cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jun 30 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (J774 macrophages)
Specification J774 macrophages
Treatment siRNA for 48h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (NIH 3T3)
Total protein in input 1e+006 cells
Specification NIH 3T3
Immuno-precipitation step Other
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Submitted Mar 22 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"