Purification notesWhole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
All lanes : Anti-SNAP23 antibody (ab50357) at 1/1000 dilution
Lane 1 : NIH3T3 whole cell extract Lane 2 : 3T3-L1 whole cell extract Lane 3 : NIH3T3 whole cell extract with immunizing peptide at 5 µg/ml
Secondary Goat Anti-Rabbit IgG, Peroxidase conjugate developed using the ECL technique
Predicted band size : 23 kDa Observed band size : 23 kDa
References for Anti-SNAP23 antibody (ab50357)
This product has been referenced in:
Hommel A et al. The ARF-like GTPase ARFRP1 is essential for lipid droplet growth and is involved in the regulation of lipolysis. Mol Cell Biol30:1231-42 (2010).
Read more (PubMed: 20038528) »
Panaretakis T et al. Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death. EMBO J28:578-90 (2009).
Read more (PubMed: 19165151) »