The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 4 µg/ml.
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 23 kDa).
Use a concentration of 5 µg/ml.
Functiont-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Tissue specificityNeurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Sequence similaritiesBelongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains.
Post-translational modificationsPalmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
Cellular localizationCytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
ICC/IF image of ab31281 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31281 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat (ab96931) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab31281 at 2.5µg/ml staining SNAP25 in human cerebral cortex tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
References for Anti-SNAP25 antibody (ab31281)
This product has been referenced in:
Enriquez-Algeciras M et al. Deimination restores inner retinal visual function in murine demyelinating disease. J Clin InvestN/A:N/A (2013).
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