Anti-SNAP25 antibody (ab5666)
Key features and details
- Rabbit polyclonal to SNAP25
- Suitable for: ICC, WB, Flow Cyt
- Reacts with: Mouse, Rat
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SNAP25 antibody
See all SNAP25 primary antibodies -
Description
Rabbit polyclonal to SNAP25 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Human, Chimpanzee, Rhesus monkey, Goldfish -
Immunogen
Synthetic peptide corresponding to Mouse SNAP25 aa 195-206.
Sequence:ANQRATKMLGSG
(Peptide available asab5843) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
1/100.
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WB | (4) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa.
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Flow Cyt |
Use 3-5µg for 106 cells.
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Notes |
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ICC
1/100. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa. |
Flow Cyt
Use 3-5µg for 106 cells. |
Target
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Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. -
Tissue specificity
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. -
Sequence similarities
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
Post-translational
modificationsPalmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. -
Cellular localization
Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 458096 Chimpanzee
- Entrez Gene: 6616 Human
- Entrez Gene: 20614 Mouse
- Entrez Gene: 25012 Rat
- Entrez Gene: 574204 Rhesus monkey
- Omim: 600322 Human
- SwissProt: Q5R1X1 Chimpanzee
- SwissProt: P36978 Goldfish
see all -
Alternative names
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
see all
Images
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All lanes : Anti-SNAP25 antibody (ab5666)
Lane 1 : Mouse cerebellum
Lane 2 : Mouse brain
Lane 3 : Rat brain
Lane 4 : Membrane enriched extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate -
ICC/IF image of ab5666 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunlocalization of SNAP-25 in cultured rat hippocampal cells using ab5666.
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Anti-SNAP25 antibody (ab5666) at 1/10000 dilution + Whole tissue lysate prepared from mouse hippocampus.
Secondary
HRP conjugated goat anti-rabbit Ig. at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (51)
ab5666 has been referenced in 51 publications.
- Lakkaraju AKK et al. Glial activation in prion diseases is selectively triggered by neuronal PrPSc. Brain Pathol 32:e13056 (2022). PubMed: 35178783
- Fan S et al. Ceftriaxone Suppresses Group II Metabotropic Glutamate Receptor Expression Contributing to Reversal of Recognition Memory Deficits of Amyloid Precursor Protein/Presenilin 1 AD Mice. Front Neurosci 16:905403 (2022). PubMed: 35860293
- Mazzo F et al. Metabotropic Glutamate Receptors Modulate Exocytotic Tau Release and Propagation. J Pharmacol Exp Ther 383:117-128 (2022). PubMed: 36116796
- Huang Q et al. SNAP25 Inhibits Glioma Progression by Regulating Synapse Plasticity via GLS-Mediated Glutaminolysis. Front Oncol 11:698835 (2021). PubMed: 34490096
- Kim K et al. Reduced Interaction of Aggregated a-Synuclein and VAMP2 by Environmental Enrichment Alleviates Hyperactivity and Anxiety in a Model of Parkinson's Disease. Genes (Basel) 12:N/A (2021). PubMed: 33801790