Overview

  • Product nameAnti-SNAP25 antibody [EP3274]
    See all SNAP25 primary antibodies
  • Description
    Rabbit monoclonal [EP3274] to SNAP25
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • Positive control
    • Fetal brain, HeLa, SH-SY5Y, U87-MG, fetal hippocampus, fetal cerebellum, and Neuro-2a cell lysates; Human brain tissue; Human cerebellum tissue.
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • Functiont-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
    • Tissue specificityNeurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
    • Sequence similaritiesBelongs to the SNAP-25 family.
      Contains 2 t-SNARE coiled-coil homology domains.
    • Post-translational
      modifications
      Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
    • Cellular localizationCytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA416N4.2 antibody
      • Bdr antibody
      • CMS18 antibody
      • dJ1068F16.2 antibody
      • FLJ23079 antibody
      • HGNC:11132 antibody
      • MGC105414 antibody
      • MGC139754 antibody
      • Resistance to inhibitors of cholinesterase 4 homolog antibody
      • RIC 4 antibody
      • RIC4 antibody
      • SEC 9 antibody
      • SEC9 antibody
      • SNAP 25 antibody
      • SNAP antibody
      • SNAP-25 antibody
      • SNAP-25B antibody
      • SNAP25 antibody
      • SNP 25 antibody
      • SNP25 antibody
      • SNP25_HUMAN antibody
      • sp antibody
      • SUP antibody
      • Super protein antibody
      • Synaptosomal associated 25 kDa protein antibody
      • Synaptosomal associated protein antibody
      • Synaptosomal associated protein 25 antibody
      • Synaptosomal associated protein 25kDa antibody
      • Synaptosomal-associated 25 kDa protein antibody
      • Synaptosomal-associated protein 25 antibody
      • Synaptosomal-associated protein, 25-KD antibody
      see all

    Anti-SNAP25 antibody [EP3274] images

    • All lanes : Anti-SNAP25 antibody [EP3274] (ab108990) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : U87-MG cell lysate
      Lane 5 : Fetal hippocampus lysate
      Lane 6 : Fetal cerebellum lysate
      Lane 7 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 23 kDa
    • ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
    • ab108990 showing negative staining in Normal colon tissue.

    • ab108990 showing negative staining in Normal spleen tissue.

    • ab108990 showing negative staining in Skeletal muscle tissue.

    References for Anti-SNAP25 antibody [EP3274] (ab108990)

    ab108990 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab108990.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"