Anti-SNAP45 antibody (ab23467)

Overview

  • Product nameAnti-SNAP45 antibody
    See all SNAP45 primary antibodies
  • Description
    Rabbit polyclonal to SNAP45
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: STEEDFAVDF EKIYKYLSSV SRSGRSPELS AAESAVVLDL LMSLPEELPL, corresponding to internal sequence amino acids 197-246 of Human SNAP45

  • Positive control
    • Jurkat cell lysate for WB. Paraffin embedded human kidney cells and epithelial cells of renal tubule for IHC.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab23467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 36 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionPart of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Proximal sequence element binding transcription factor delta subunit antibody
    • Proximal sequence element binding transcription factor subunit delta antibody
    • Proximal sequence element-binding transcription factor subunit delta antibody
    • PSE binding factor delta subunit antibody
    • PSE binding factor subunit delta antibody
    • PSE-binding factor subunit delta antibody
    • PTF delta subunit antibody
    • PTF subunit delta antibody
    • PTFdelta antibody
    • Small nuclear RNA activating complex polypeptide 2 antibody
    • Small nuclear RNA-activating complex polypeptide 2 antibody
    • SNAP45 antibody
    • SNAPC 2 antibody
    • SNAPc 45 kDa subunit antibody
    • SNAPc subunit 2 antibody
    • Snapc2 antibody
    • SNPC2_HUMAN antibody
    • snRNA activating protein complex 2 antibody
    • snRNA activating protein complex 45 kDa subunit antibody
    • snRNA activating protein complex subunit 2 antibody
    • snRNA-activating protein complex 45 kDa subunit antibody
    • snRNA-activating protein complex subunit 2 antibody
    see all

Anti-SNAP45 antibody images

  • Anti-SNAP45 antibody (ab23467) at 0.4 µg/ml + Jurkat cell lysate 20ug.

    Secondary
    HRP conjugated anti-rabbit IgG diluted at 1/50,000-1/100,000.

    Predicted band size : 36 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SNAP45 with ab23467 at a concentration of 4-8µg/ml. Cells with positive labels (arrows) are Epithelial cells of renal tubule.

References for Anti-SNAP45 antibody (ab23467)

ab23467 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Embryo)
Specification Embryo
Fixative Paraformaldehyde
Permeabilization Yes - Triton-X-1oo
Blocking step Donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Jul 02 2012

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