The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceSNAP47 belongs to the SVAP1 family. It contains two t-SNARE coiled-coil homology domains.
SNAP47 has ubiquitous tissue distribution, with particularly high levels in nervous tissue. In neurons, SNAP 47 has been shown to have widespread distribution on intracellular membranes and is enriched in synaptic vesicle fractions. In vitro, SNAP 47 can substitute for SNAP-25 in SNARE complex formation, but not as efficiently.
Cellular localizationEndomembrane system. Cytoplasm; perinuclear region. Note: Appears to be exclusively membrane-bound.