Recombinant
RabMAb

Anti-SNAP47 antibody [EPR12313] (ab172609)

Overview

  • Product name
    Anti-SNAP47 antibody [EPR12313]
    See all SNAP47 primary antibodies
  • Description
    Rabbit monoclonal [EPR12313] to SNAP47
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SNAP47. The exact sequence is proprietary.
    Database link: Q5SQN1

  • Positive control
    • Fetal brain, Human cerebellum, U87-MG and HepG2 lysates; HepG2 cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 0.05% BSA, 50% Glycerol
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12313
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 52 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Relevance
      SNAP47 belongs to the SVAP1 family. It contains two t-SNARE coiled-coil homology domains. SNAP47 has ubiquitous tissue distribution, with particularly high levels in nervous tissue. In neurons, SNAP 47 has been shown to have widespread distribution on intracellular membranes and is enriched in synaptic vesicle fractions. In vitro, SNAP 47 can substitute for SNAP-25 in SNARE complex formation, but not as efficiently.
    • Cellular localization
      Endomembrane system. Cytoplasm; perinuclear region. Note: Appears to be exclusively membrane-bound.
    • Database links
    • Alternative names
      • C1orf142 antibody
      • Chromosome 1 open reading frame 142 antibody
      • FLJ12517 antibody
      • HEL170 antibody
      • OTTHUMP00000035832 antibody
      • SNAP 47 antibody
      • SVAP1 antibody
      • Synaptosomal associated protein, 47kDa antibody
      • t SNARE coiled coil homology domain containing protein C1orf142 antibody
      see all

    Images

    • All lanes : Anti-SNAP47 antibody [EPR12313] (ab172609) at 1/1000 dilution

      Lane 1 : Fetal brain tissue lysate
      Lane 2 : Human cerebellum tissue lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 52 kDa

    • Flow cytometric analysis of permeabilized HepG2 cells labeling SNAP47 with ab172609 at 1/100 dilution (red) compared to a Rabbit IgG negative control (green).

    References

    ab172609 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab172609.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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