Overview

  • Product name
  • Description
    Rabbit polyclonal to SNAPC3
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: MAEGSRGGPT CSGVGGRQDP VSGSGGCNFP EYELPELNTR AFHVGAFGEL, corresponding to a region within N terminal amino acids 1-50 of Human SNAPC3

  • Positive control
    • Fetal small intestine lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Part of the SNAPc complex required for the transcription of both RNA polymerase II and III small-nuclear RNA genes. Binds to the proximal sequence element (PSE), a non-TATA-box basal promoter element common to these 2 types of genes. Recruits TBP and BRF2 to the U6 snRNA TATA box.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MGC132011 antibody
    • MGC33124 antibody
    • Proximal sequence element-binding transcription factor subunit beta antibody
    • PSE binding factor subunit beta antibody
    • PSE-binding factor subunit beta antibody
    • PTF subunit beta antibody
    • PTFbeta antibody
    • small nuclear RNA activating complex, polypeptide 3, 50kDa antibody
    • Small nuclear RNA-activating complex polypeptide 3 antibody
    • SNAP50 antibody
    • SNAPc 50 kDa subunit antibody
    • SNAPc subunit 3 antibody
    • SNAPC3 antibody
    • SNPC3_HUMAN antibody
    • snRNA activating protein complex 50 kDa subunit antibody
    • snRNA activating protein complex subunit 3 antibody
    • snRNA-activating protein complex 50 kDa subunit antibody
    • snRNA-activating protein complex subunit 3 antibody
    see all

Images

  • Anti-SNAPC3 antibody (ab66328) at 2.5 µg/ml (in 5% skim milk / PBS buffer) + Fetal small intestine lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

References

ab66328 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66328.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up