Overview

  • Product nameAnti-SNAPIN antibody
    See all SNAPIN primary antibodies
  • Description
    Mouse monoclonal to SNAPIN
  • Tested applicationsSuitable for: ELISA, WB, IHC-P, Sandwich ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of Human SNAPIN (amino acids 41-136) with N terminal proprietary tag, 11kDa (without tag). (NP_036569).

  • Positive control
    • Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab118392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 15 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.
  • Tissue specificityExpressed in male germ cells of adult testis (at protein level).
  • Sequence similaritiesBelongs to the SNAPIN family.
  • Developmental stageExpressed in germ cells of 22-week prenatal testis.
  • Cellular localizationCytoplasm. Membrane. Cytoplasmic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Cytoplasm > perinuclear region. May be cytoplasmic and peripheral membrane bound or anchored to the vesicular membrane through an N-terminal signal anchor (By similarity). Co-localizes with NANOS1 and PUM2 in the perinuclear region of germ cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000035157 antibody
    • SNAP associated protein antibody
    • SNAP-25-binding protein antibody
    • SNAP-associated protein antibody
    • SNAP25BP antibody
    • SNAPAP antibody
    • Snapin antibody
    • SNAPN_HUMAN antibody
    • SNARE associated protein snapin antibody
    • SNARE-associated protein Snapin antibody
    • Synaptosomal associated protein 25 binding protein antibody
    • Synaptosomal-associated protein 25-binding protein antibody
    see all

Anti-SNAPIN antibody images

  • Overlay histogram showing HeLa cells stained with ab118392 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118392, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-SNAPIN antibody (ab118392) at 5 µg/ml + recombinant immunogen at 0.2 µg

    Predicted band size : 15 kDa
    Western blot against tagged recombinant immunogen using ab118392 antibody at 5µg/ml dilution. Predicted band size of immunogen is 36.67kDa.
  • ab118392, at 3 ug/ml, staining SNAPIN in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.
  • Detection limit for recombinant tagged SNAPIN is 1 ng/ml as a capture antibody.

References for Anti-SNAPIN antibody (ab118392)

ab118392 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"