The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 70 kDa for the fusion protein (predicted molecular weight for the native protein: 15 kDa).
FunctionMay modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release by potentiating the interaction of synaptotagmins with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells.
Tissue specificityExpressed in male germ cells of adult testis (at protein level).
Sequence similaritiesBelongs to the SNAPIN family.
Developmental stageExpressed in germ cells of 22-week prenatal testis.
Cellular localizationCytoplasm. Membrane. Cytoplasmic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Cytoplasm > perinuclear region. May be cytoplasmic and peripheral membrane bound or anchored to the vesicular membrane through an N-terminal signal anchor (By similarity). Co-localizes with NANOS1 and PUM2 in the perinuclear region of germ cells.