Overview

  • Product name
  • Description
    Mouse polyclonal to SNF2H
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Full length protein corresponding to amino acids 1-1052 of Human SNF2H (AAH23144.1).

  • Positive control
    • SNF2H transfected 293T cell lysate and Human liver tissue lysate; HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 122 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 SANT domains.
  • Developmental stage
    Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4933427E24Rik antibody
    • D030040M08Rik antibody
    • D330027N15Rik antibody
    • EC 3.6.1.- antibody
    • hISWI antibody
    • hSNF2H antibody
    • ISWI antibody
    • MommeD4 antibody
    • SMARCA5 antibody
    • SMCA5_HUMAN antibody
    • Snf2h antibody
    • Sucrose nonfermenting like 5 antibody
    • Sucrose nonfermenting protein 2 homolog antibody
    • Sucrose nonfermenting, yeast, homolog of antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 antibody
    • WCRF135 antibody
    see all

Images

  • All lanes : Anti-SNF2H antibody (ab168653) at 1 µg/ml

    Lane 1 : SNF2H transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate


    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP
    at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 122 kDa

  • Anti-SNF2H antibody (ab168653) at 1/500 dilution + Human liver tissue lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP
    at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 122 kDa

  • Immunofluorescence analysis of HeLa cells, labeling SNF2H with ab168653 at 10 µg/ml.

References

ab168653 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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