• Product nameAnti-SNF2H antibody
    See all SNF2H primary antibodies
  • Description
    Mouse monoclonal to SNF2H
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: EEIFDDASPG KQKEIQEPDP TYEEKMQTDR ANRFEYLLKQ TELFAHFIQP AAQKTPTSPL KMKPGRPRIK KDEKQNLLSV GDYRHRRTE, corresponding to amino acids 59-148 of Human SNF2H (NP_003592) with tag.

  • Positive control
    • Recombinant tagged SNF2H fragment.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab76459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged SNF2H is approximately 3ng/ml if used as a capture antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 122 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.


  • FunctionHelicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. ISWI subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 SANT domains.
  • Developmental stageOverexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4933427E24Rik antibody
    • D030040M08Rik antibody
    • D330027N15Rik antibody
    • EC 3.6.1.- antibody
    • hISWI antibody
    • hSNF2H antibody
    • ISWI antibody
    • MommeD4 antibody
    • SMARCA5 antibody
    • SMCA5_HUMAN antibody
    • Snf2h antibody
    • Sucrose nonfermenting like 5 antibody
    • Sucrose nonfermenting protein 2 homolog antibody
    • Sucrose nonfermenting, yeast, homolog of antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 antibody
    • WCRF135 antibody
    see all

Anti-SNF2H antibody images

  • Anti-SNF2H antibody (ab76459) at 1 µg/ml + Recombinant tagged SNF2H fragment at 0.1 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 122 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References for Anti-SNF2H antibody (ab76459)

ab76459 has not yet been referenced specifically in any publications.

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