For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide derived from residues 1000 to the C-terminus of Human SNF2H.
Our Abpromise guarantee covers the use of ab3749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 16917504|
|ChIP||Use 2.5µl for 106 cells.|
|WB||1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa).|
Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.
Lane 1: HeLa lysate (20ug)
Lane 2: Mouse 3T3 lysate (20ug)
Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)
ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immunising peptide ab5160.
ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.
Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estro
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"