Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab3749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 16917504
ICC/IF 1/500.
ChIP Use 2.5µl for 106 cells.
WB 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa).

Target

  • FunctionHelicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the SNF2/RAD54 helicase family. ISWI subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 SANT domains.
  • Developmental stageOverexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4933427E24Rik antibody
    • D030040M08Rik antibody
    • D330027N15Rik antibody
    • EC 3.6.1.- antibody
    • hISWI antibody
    • hSNF2H antibody
    • ISWI antibody
    • MommeD4 antibody
    • SMARCA5 antibody
    • SMCA5_HUMAN antibody
    • Snf2h antibody
    • Sucrose nonfermenting like 5 antibody
    • Sucrose nonfermenting protein 2 homolog antibody
    • Sucrose nonfermenting, yeast, homolog of antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 antibody
    • WCRF135 antibody
    see all

Anti-SNF2H antibody - ChIP Grade images



  • Predicted band size : 122 kDa


    Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.

    Lane 1: HeLa lysate (20ug)
    Lane 2: Mouse 3T3 lysate (20ug)
    Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
    Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug

    Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)

    ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immunising peptide ab5160.
  •  

    ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.

      

    Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers were designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 2.5 µl of ab3749 and 2x106 cells were used in each ChIP experiment.

     
     
     
  • Interphase HeLa cells immunofluorescently labeled with anti-SNF2H ab3749 (1/500). This image shows a strong and predominantly nuclear localisation of SNF2H. Secondary antibody used: polyclonal goat anti-rabbit antibody (1/200) conjugated to Cy3.

    See Abreview

References for Anti-SNF2H antibody - ChIP Grade (ab3749)

This product has been referenced in:
  • Wille A  et al. Impaired Contextual Fear Extinction Learning is Associated with Aberrant Regulation of CHD-Type Chromatin Remodeling Factors. Front Behav Neurosci 9:313 (2015). WB ; Mouse . Read more (PubMed: 26635563) »
  • Dluhosova M  et al. Epigenetic control of SPI1 gene by CTCF and ISWI ATPase SMARCA5. PLoS One 9:e87448 (2014). ChIP ; Human . Read more (PubMed: 24498324) »

See all 22 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Human Cell (BPH1 prostate cells)
Specification BPH1 prostate cells
Permeabilization Yes - Triton 100
Fixative Formaldehyde
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Submitted Jul 30 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Sample Human Cell (293T)
Specification 293T
Permeabilization Yes - 0.5% Triton X100
Fixative Methanol
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Submitted Jun 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (12% SDS-PAGE)
Sample Human Cell lysate - nuclear (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

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Verified customer

Submitted Sep 05 2013

This would be an appropriate incubation solution for use with this antibody. We recommend use of this item at a starting concentration range near 1/500 and to incubate overnight at +4C.

Thank you for contacting us. As discussed on phone we unfortunately do not sell small sizes of our products. The reason is that; we have over 70,000 products in our catalogue and having the small sized sample of each would be difficult to keep. How...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HeLa cells)
Loading amount 20 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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Submitted May 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - nuclear (HeLa)
Total protein in input 200 µg
Specification HeLa
Immuno-precipitation step Protein A/G
Username

Dr. chee-kwee ea

Verified customer

Submitted Apr 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (osteosarcoma)
Loading amount 20 µg
Specification osteosarcoma
Treatment siRNA transfected with control and hsnf2h siRNA
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Sonia Rocha

Verified customer

Submitted Jul 17 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A549)
Loading amount 100000 cells
Specification A549
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (Thymus, fibroblasts, hek293, jurkat)
Specification Thymus, fibroblasts, hek293, jurkat
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step Real-time PCR
Positive control Thymus, fibroblasts, hek293, and jurkat cells
The real time PCR primers amplified different regions of the hCD2 LCR
Negative control Thymus, fibroblasts, hek293, and jurkat cells
Primers amplifying the HPRT gene locus were used as a negative control
Username

Dr. Eleni Ktisstaki

Verified customer

Submitted Nov 07 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"