Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab3749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 16917504
ICC/IF 1/500.
ChIP Use 2.5µl for 106 cells.
WB 1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa).

Target

  • Function
    Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 2 SANT domains.
  • Developmental stage
    Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4933427E24Rik antibody
    • D030040M08Rik antibody
    • D330027N15Rik antibody
    • EC 3.6.1.- antibody
    • hISWI antibody
    • hSNF2H antibody
    • ISWI antibody
    • MommeD4 antibody
    • SMARCA5 antibody
    • SMCA5_HUMAN antibody
    • Snf2h antibody
    • Sucrose nonfermenting like 5 antibody
    • Sucrose nonfermenting protein 2 homolog antibody
    • Sucrose nonfermenting, yeast, homolog of antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5 antibody
    • WCRF135 antibody
    see all

Anti-SNF2H antibody - ChIP Grade images

  • Interphase HeLa cells immunofluorescently labeled with anti-SNF2H ab3749 (1/500). This image shows a strong and predominantly nuclear localisation of SNF2H. Secondary antibody used: polyclonal goat anti-rabbit antibody (1/200) conjugated to Cy3.

    See Abreview

  •  

    ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.

      

    Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estro

References for Anti-SNF2H antibody - ChIP Grade (ab3749)

This product has been referenced in:
  • Almuzzaini B  et al. In ß-actin knockouts, epigenetic reprogramming and rDNA transcription inactivation lead to growth and proliferation defects. FASEB J 30:2860-73 (2016). Read more (PubMed: 27127100) »
  • Wang Y  et al. SNF2H promotes hepatocellular carcinoma proliferation by activating the Wnt/ß-catenin signaling pathway. Oncol Lett 12:1329-1336 (2016). WB ; Human . Read more (PubMed: 27446433) »

See all 32 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Human Cell (BPH1 prostate cells)
Specification
BPH1 prostate cells
Permeabilization
Yes - Triton 100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 30 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Sample
Human Cell (293T)
Specification
293T
Permeabilization
Yes - 0.5% Triton X100
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Jun 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (12% SDS-PAGE)
Sample
Human Cell lysate - nuclear (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted Sep 05 2013

This would be an appropriate incubation solution for use with this antibody. We recommend use of this item at a starting concentration range near 1/500 and to incubate overnight at +4C.

Thank you for contacting us. As discussed on phone we unfortunately do not sell small sizes of our products. The reason is that; we have over 70,000 products in our catalogue and having the small sized sample of each would be difficult to keep. How...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (6%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (HeLa)
Total protein in input
200 µg
Specification
HeLa
Immuno-precipitation step
Protein A/G
Username

Dr. Chee-Kwee Ea

Verified customer

Submitted Apr 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (osteosarcoma)
Loading amount
20 µg
Specification
osteosarcoma
Treatment
siRNA transfected with control and hsnf2h siRNA
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Sonia Rocha

Verified customer

Submitted Jul 17 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A549)
Loading amount
100000 cells
Specification
A549
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 26 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - nuclear (Thymus, fibroblasts, hek293, jurkat)
Specification
Thymus, fibroblasts, hek293, jurkat
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Positive control
Thymus, fibroblasts, hek293, and jurkat cells
The real time PCR primers amplified different regions of the hCD2 LCR
Negative control
Thymus, fibroblasts, hek293, and jurkat cells
Primers amplifying the HPRT gene locus were used as a negative control
Username

Dr. Eleni Ktisstaki

Verified customer

Submitted Nov 07 2006

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up